Figure 9 . Annotation and distribution of DEGs
(|log2FC| > 2, p value < 0.05)
in the pathway of protein processing in the endoplasmic reticulum ofM. rosenbergii . KEGG enrichment of DEGs in up-regulated
expression in the brain (A) and up-regulated expression in the thoracic
ganglion (B). The DEGs were separated into different principal
components (C) the distribution is shown (D) according to tissues and
sexes of M. rosenbergii . FB: female brain; FG: female thoracic
ganglion; MB: male brain; MG: male thoracic ganglion; M: male; F:
female.
The DEGs were involved in protein recognition by luminal chaperones
(k09486, 3 genes), polypeptide folding, and correction (k1718, 3 genes).
Most of the DEGs presented in the ER-associated degradation pathway
(ERAD), a process which starts with misfolded protein recognition,
followed by ubiquitination and retrotranslocation into the cytosol for
degradation in proteasome (Figure 10). Protein disulfide- isomerases
(PDIs, K09580, 10 genes) and Osteosarcoma-9 (OS-9, k10088, 1 gene)
delivered misfolded polypeptide to the ubiquitin ligase complex, which
is composed of ubiquitin-protein ligases such as Dao10 (k10661, 3
genes), Sel1L (k14026, 4 genes), and Hsp40 (k09502, 5 genes) in ERAD. A
translocon-associated protein, TRAP (k13251, 3 genes), showed
up-regulated expression in the male brain and thoracic ganglion, which
plays a role in accelerating ER degradation of unfolded proteins (Figure
10).