3.6 Validation of differentially expressed genes
To validate the DEGs, ten genes were selected using a qRT-PCR method, to determine their relative expression levels. These genes includedSmc6 (structural maintenance of chromosomes protein 6) in ubiquitin protein ligase binding activity, Iagbp (insulin-like androgen gland hormone binding protein) in feedback IAG signaling,Aspm (abnormal spindle-like microcephaly associated protein homolog) in the functional regulation of neurogenesis and brain growth,fem1b , transformer2 , and female-lethal d in sex determination and differentiation, Ddb1 (DNA damage-binding protein 1) in protein processing-related E3 ubiquitin ligase complex,Hsp90b1 (heat shock protein 90 beta 1) in stabilizing and folding other proteins, Gsg2 (ganglioside GM2 activator) in reductions of GM2 gangliosidosis (mutations in this gene result in Alzheimer’s disease in humans), and alpha 2 macroglobulin gene. The gene expression results from qRT-PCR are consistent with the values of FPKM from Illumina sequencing under the same conditions (Figure 8), indicating that the DEGs from the RNA-seq data were reliable.