2.7 Construction of LncRNA and gene co-expression networks
A network of LncRNA-gene co-expressions was constructed using the Pearson correlation coefficient between the differentially expressed LncRNAs and differentially expressed mRNAs using the FPKM values of four tissue samples via Illumina sequencing. The threshold for positive correlation was set to PCC > 0.95 and P-value < 0.05. The regulatory network of LncRNAs and mRNA was established via Cytoscape (Smoot et al. 2011), and the ZFLNC database (Hu et al.2018) was used to conduct a conservative analysis of lncRNA.