3.6 Validation of differentially expressed genes
To validate the DEGs, ten genes were selected using a qRT-PCR method, to
determine their relative expression levels. These genes includedSmc6 (structural maintenance of chromosomes protein 6) in
ubiquitin protein ligase binding activity, Iagbp (insulin-like
androgen gland hormone binding protein) in feedback IAG signaling,Aspm (abnormal spindle-like microcephaly associated protein
homolog) in the functional regulation of neurogenesis and brain growth,fem1b , transformer2 , and female-lethal d in sex
determination and differentiation, Ddb1 (DNA damage-binding
protein 1) in protein processing-related E3 ubiquitin ligase complex,Hsp90b1 (heat shock protein 90 beta 1) in stabilizing and folding
other proteins, Gsg2 (ganglioside GM2 activator) in reductions of
GM2 gangliosidosis (mutations in this gene result in Alzheimer’s disease
in humans), and alpha 2 macroglobulin gene. The gene expression
results from qRT-PCR are consistent with the values of FPKM from
Illumina sequencing under the same conditions (Figure 8), indicating
that the DEGs from the RNA-seq data were reliable.