Figure 2. Distribution of unigene homologous to BUSCO database (A), annotation number of seven databases for unigenes from M. rosenbergii (B ). The abbreviated seen above.
Homologous species analysis of all unigenes annotated in the NR database showed that Hyalella azteca had the highest unigene distribution (21,754, 37.6%), followed by Cryptotermes secundus (3136, 5.42%), Procambarus clarkii (2,702, 4.67%), Zootermopsis nevadensis (2,523, 4.36%), and M. rosenbergii (1,749, 3.02%) (Figure S1). GO analysis demonstrated that 46,661 unique genes were enriched significantly in three major categories: biological process (41,379), cellular component (43,948), and molecular function (41,174). In these three categories, the top three abundant subcategories were cellular process (79.1%), metabolic process (56.1%), and biological regulation (53.6%) in biological process; cell (86.1%), cell part (85.9%), and organelle (68.4%) in cellular component; binding (71.8%), catalytic activity (41.7%), and transporter activity (7.9%) in molecular function (Figure 3).