3.2 Assessment of unigene completeness
The completeness and accuracy of the obtained unigenes were assessed via BUSCO with the arthropoda-odb9 database. Compared to 60 species with 1,066 available gene sequences in the database, 994 (93.2%) sequences were completely homologous to matches within the BUSCO database (Figure 2A). Our SMART data were determined to be of high quality and could be used for subsequent analysis.