2.7 Construction of LncRNA and gene co-expression networks
A network of LncRNA-gene co-expressions was constructed using the
Pearson correlation coefficient between the differentially expressed
LncRNAs and differentially expressed mRNAs using the FPKM values of four
tissue samples via Illumina sequencing. The threshold for positive
correlation was set to PCC > 0.95 and P-value <
0.05. The regulatory network of LncRNAs and mRNA was established via
Cytoscape (Smoot et al. 2011), and
the ZFLNC database (Hu et al.2018) was used to conduct a conservative analysis of lncRNA.