2.3. DPPH Assay Protocol
In the recent experiment, 1ml of DPPH methanol solution was added to 1ml
of concentrate to 3 ml of nanoparticles and the resulting mixture was
stirred vigorously. The test tubes were placed in a dark place for 30
minutes. After this period, the absorbance at the wavelength of 517 nm
was read. It should be noted that in the control sample, the
nanoparticles was replaced with 3 ml of methanol. Finally, the DPPH
radical’s inhibition percentage was calculated with this formula
[22,23]:
\begin{equation}
\text{Inhibition\ }\left(\%\right)=\frac{\text{Sample\ A.}}{\text{Control\ A.\ }}x100\nonumber \\
\end{equation}