2.1. Preparation and extraction of aqueous extract
To prepare the aqueous extract of Salvia officinalis , 6 g of the
dried part of the mill was poured into 150 ml of boiling water and
boiled for ten minutes. Then the extract was allowed to cool down
gradually. After complete cooling, the resulting extract was clarified
by a high-speed centrifuge (20,000 rpm) for 15 minutes. The resulting
sample was wrapped in aluminum foil and kept in the refrigerator.