2.3. DPPH Assay Protocol
In the recent experiment, 1ml of DPPH methanol solution was added to 1ml of concentrate to 3 ml of nanoparticles and the resulting mixture was stirred vigorously. The test tubes were placed in a dark place for 30 minutes. After this period, the absorbance at the wavelength of 517 nm was read. It should be noted that in the control sample, the nanoparticles was replaced with 3 ml of methanol. Finally, the DPPH radical’s inhibition percentage was calculated with this formula [22,23]:
\begin{equation} \text{Inhibition\ }\left(\%\right)=\frac{\text{Sample\ A.}}{\text{Control\ A.\ }}x100\nonumber \\ \end{equation}