2.1 Identification of antigen-binding CDRs
The structure of the EGFR DIII/Panitumumab complex (PDB entry 5SX4) was used as the initial complex for extracting the affective CDRs in interaction with the receptor. Two CDRs, H2 and H3, from the heavy chain and L3 from the light chain were selected for grafting into three scaffolds of Lipocalin (PDB entry 3dwt), Fn3 (1TTF), and VHH (3BX7). The sequence of these CDRs defined by Parapred software [10], each with two residues per site (residues on the upper and lower core of variable domains affecting CDRs conformation) [9].